Vanderbilt University Medical Center Research Assistant I - Neurology in Nashville, Tennessee
Recruiting for a Research Assistant I as part of the Division of Epilepsy within the Department of Neurology at Vanderbilt University Medical Center. Our lab is broadly interested in mechanisms of seizure related breathing disturbances and epileptogenesis. Reporting directly to the Principal Investigator the Research Assistant will interact with faculty, staff, and students within the laboratory. The RA I would be expected to perform experiments under moderate supervision and direction from the PI as well as assist in managing the lab. This would entail but is not limited to management of mouse colony and breeding, assisting in stereotaxic mouse surgeries and post-operative care, dissection of mouse tissues, operating a cryostat for generation of tissues sections, immunohistochemistry, and fluorescent/confocal microscopy to capture images of neurons, isolation of RNA and RT-PCR. Project responsibilities will also include working with mouse lines and maintaining a breeding colony including DNA isolation, genotyping, and timed matings, animal surgery preparation and operation.
Ability to work with others and individually.
Reliable and professional attitude.
Eager – Enthusiastic, wanting to learn, wanting to continueone’s personal and professional development and expertise.
Experience with the care of rodents and with the observationof rodent behaviors.
Experiencewith Wet Lab Experiments:
Perform calculations involving molarities, % solutions,serial dilutions, and statistical analysis of experimental results.
Participate with maintenance of mouse breeding colony,including performing crosses, DNA isolation, and genotyping.
Experience with dissection of mouse tissues,immunohistochemistry, and fluorescent microscopy
Any experience with stereotactic mouse surgeries appreciatedbut not required
Experience with molecular techniques such as PCR, genotyping,and cataloging of transgenic mice; experience with standard techniques ofwestern blotting, immunocytochemistry and immunoblotting.
Bachelor's Degree (or equivalent experience) and < 1 year experience
Licensure, Certification, and/or Registration (LCR):
Physical Requirements/Strengths needed & Physical Demands:
Occasional: Sitting: Remaining in seated position
Occasional: Standing: Remaining on one's feet without moving.
Occasional: Walking: Moving about on foot.
Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another
Occasional: Push/Pull: Exerting force to move objects away from or toward.
Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles
Occasional: Crawling:Moving about on hands and knees or hands and feet.
Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.
Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.
Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.
Occasional: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.
Continuous: Communication: Expressing or exchanging written/verbal/electronic information.
Occasional: Chemicals and Gasses: Medications, cleaning chemicals, oxygen, other medical gases used in work area.
Occasional: Pathogens: Risk of exposure to bloodborne pathogens and other contagious illnesses.
Occasional: Radiation: May be exposed to occupational radiation, requiring enrollment in VUMC's Radiation Safety Program which includes training, use of personal protective equipment with lead shielding, and personal dose monitoring.
Occasional: Climate: Ability to withstand exposure to atmospheric extremes including heat, cold, humidity, and barometric pressure changes.
Organization: Neurology-Macdonald 104401
Title: Research Assistant I - Neurology
Location: TN-Nashville-MRB III BIO/SCI
Requisition ID: 1811023
Vanderbilt University Medical Center is committed to principles of equal opportunity and affirmative action.